高效液相色谱法测定间苯二酚反应液中间苯二酚的含量

ANALYSIS OF RESORCINOL IN REACTION LIQUID BY HPLC

建立了高效液相色谱法测定间苯二酚反应液中间苯二酚含量的方法。色谱柱为Diamonsil ODS（150mm×4．6mm，5um），流动相为A与B的混合液（体积比为5：95，A：乙腈，B：5mmol／L 1，8-二胺辛烷与20mmol/L庚磺酸钠水溶液混合后调节至pH4．5），检测波长为276nm。间苯二酚与反应液中其它杂质分离较好。间苯二酚的浓度在0．1～0．5g/L范围内与色谱峰面积呈良好的线性。加标回收率为99．25％～99．44％，测定结果的相对标准偏差为0．85％（n=8）。

The content of resorcinol in resoreinol reaction liquid was determined by HPLC. The column was Diamonsil ODS （ 150 mm × 4.6 mm, 5 um）, and the mobile phase was A - B mixture solution（ volume ratio was 5 ： 95, A ： ACN, B ：5 mmol/L 1,8- Diamino - octane and 20 mmol/L sodium 1-heptanesulfonate, pH 4.5）. The detection wavelength was 276 nm. The concentration of resorcinol had linear relationship with peak area in th range of 0.1 - 0.5 g/L. The recovery was 99.25% - 99.44%. The relative devation of detection results was 0.85% （n = 8）.