新型β-双链桥连双卟啉的合成及光敏活性

Synthesis of Novel β-Double-bridged Porphyrin Dimers and Their Photosensitive Activity Investigation

设计合成了一种β-双链桥连双卟啉及其Cu(Ⅱ),Ni(Ⅱ),Zn(Ⅱ)配合物,通过1HNMR,MS,IR,UV和元素分析进行了表征.以1,3-二苯基异苯并呋喃(DPBF)为猝灭剂测试了β-双链双卟啉产生单线态氧的能力;通过DNA凝胶电泳比较了其对pBR322质粒DNA的光敏切割效果;采用紫外-可见光谱滴定法研究了其与小牛胸腺DNA的相互作用.实验结果表明,光敏剂β-双链双卟啉具有较强的产生单线态氧能力,β-双链桥连使双卟啉对pBR322质粒DNA具有较好的光敏切割效果,与DNA有较强的结合能力.

Four novel β-double-bridged porphyrin dimers were designed and synthesized for the purpose of their photosensitivity and structure-activity relationship study. Their structures were characterized by UV, 1 H NMR,IR,MS and elementary analysis. The ability of singlet oxygen production for the porphyrin dimmers under photo irradiation were determined with DPBF as the quencher. Its photosensitive cleavage ability to pBR322 plasmid DNA has been tested by gel electrophoresis. The interaction of the porphyrin dimer with CT DNA was detected by UV-Vis spectroscopy. The results showed that novel β-double-bridged porphyrin dimers exhibit obvious photosensitive cleavage activity on DNA,resulting from its well binding effects,which is better than that of β-single-bridged porphyrin dimer correspondly.