基于锁核酸的高选择性新型分子信标

Novel Molecular Beacon with High Selectivity Based on Locked Nucleic Acids

通过在分子信标的错配位点修饰锁核酸,不仅可有效地改善其单碱基错配识别能力,还可提高检测灵敏度.因而有望发展成为一种通用的提高分子信标单碱基错配识别能力的方法.

A novel kind of molecular beacon which was modified using locked nucleic acids at mismatch sites was reported. Hybridization kinetics of different molecular beacons with cDNA and single-base mismatch DNA,and melting temperature were investigated using fluorescence spectroscopy. The results showed that molecular beacon with only the mismatch site replaced with LNA had the highest single-base mismatch discrimination capability. Such novel molecular beacon could not only improve effectively single-base mismatch recognition ability,but also enhance the sensitivity. Probably,it was resulted by the high-affinity of locked nucleic acid. Therefore,it was expected to develop a common method for improving the single-base mismatch recognition ability.