血必净对心肺复苏后转录因子GATA-3和T-bet表达调节的实验研究

The effects of Xuebijing injection on the expressions of transcription factors T-bet and GATA-3 of the rats after cardiopulmonary resuscitation

目的 探讨血必净对心肺复苏后转录因子GATA-3和T-bet表达的调节作用.方法 建立心肺复苏大鼠模型,30只SD大鼠动物随机（随机数字法）分为三组：假手术组（B组）,仅进行麻醉和气管切开插管、血管穿刺,不进行窒息及心肺复苏;常规复苏组（C组）,常规复苏＋生理盐水4 mL/kg;血必净治疗组（D组）,常规复苏＋血必净4mL/kg.动态观察血清GATA-3和T-betmRNA表达及白介素-12（IL-12）、白介素-4（IL-4）、r-干扰素（IFN-γ）、肿瘤坏死因子-α（TNF-α）水平的变化.多组间均数比较采用单因素方差分析,方差齐则采用成组设计资料的t检验,两两比较用SNK法;如方差不齐,则采用随机区组设计的秩和检验（Wilcoxon检验）,P〈0.05为差异有统计学意义.结果 C,D组复苏后6 h与对照组、复苏后即刻比较,IL-12,IFN-γ,TNF-α水平均升高（P〈0.01）;C组复苏后6 h GATA表达下调（P〉0.05）,T-bel表达上调（P〈0.01）,GATA-3/T-bet下降（P〈0.05）.D组复苏后6 h T-bet表达上调（P〉0.05）,GATA表达上调（P〈0.01）,GATA-3/T-bet升高（P〈0.05）;D组复苏后6h与C组同时点比较,IL-12,IFN-γ,TNF-α水平低于C组（P〈0.01）,GATA-3表达上调、T-bet表达下调、GATA-3/T-bet升高均有统计学意义（P〈0.01）.结论 心肺复苏后存在转录因子T-bet和GATA-3失衡表达,血必净可调节GATA-3/T-bet趋向平衡及细胞因子IL-12,IFN-γ,TNF-α水平.

Objective To investigate the effects of Xuebijing injection on the transcription factors GATA-3 and T-bet of rats after cardiopulmonary resuscitation （CPR）. Method The rat models of CPR was made by using asphyxia method. Thirty SD rats were randomly （random number） divided into three groups at random： sham operation rats （B group）, conventional CPR rats （C group） and Xuebijing （4 mL/kg） treated rats （D group）. The levels of serum IL-12, IL-4, TNF-α and IFN-γ were measured by using ELISA. The expressions of T-bet and GATA-3 mRNA in serum were detected by RT-PCR. The analysis of variance was used to compare the means of different groups including t-test and Wilcoxon test. Results Compared with group B, the levels of serum IL-12, TNF-α and IFN-γ in group C and group D were significantly elevated after CPR for 6 hours （P〈0.01）. In group C, the expression of GATA-3 mRNA and GATA-3/T-bet decreased （both P 〈 0.05）, while the expression of T-bet mRNA increased （P〈0.01） after CPR for 6 hours. In group D, the expressions of GATA-3 mRNA and T-bet mRNA as well as GATA-3/T-bet increased after CPR for 6 hours. The levels of IL-12, IFN-γ and TNF-α in group D were lower than those in groupC （P〈0.01）. Compared with group C, the expression of GATA-3 mRNA and GATA-3/T-bet were significantly elevated and the expression of T-bet mRNA decreased in group D. ConclusionsThe transcription factors GATA-3 and T-bet may fail to get balance after CPR. The Xiebijing injectio can modulate the balance between GATA-3 and T-bet, and the levels of IL-12,IFN-γ and TNF-α.