白藜芦醇对中波紫外线照射致成纤维细胞氧化损伤的影响

Resveratrol protects against oxidative damage of fibroblasts irradiated with UVB

目的探讨白藜芦醇对中波紫外线（UVB）照射致体外培养人皮肤成纤维细胞氧化损伤的预防和治疗作用。方法实验分为5组：正常对照组、白藜芦醇组、UVB照射组、UVB照射后白藜芦醇处理组及白藜芦醇预处理后UVB照射组。细胞处理后用甲基噻唑基四唑（MTT）法检测细胞增殖活性，酶生化法检测细胞超氧化物歧化酶（SOD）活性、丙二醛（MDA）含量。结果自藜芦醇浓度〉100μmol／L时可抑制成纤维细胞增殖，〈100μmol／L时可促进成纤维细胞增殖，最佳作用浓度为50μmol／L；UVB照射后细胞活性下降31．7％，细胞SOD活性降低、MDA产生增加；与单纯UVB照射相比，UVB照射前或照射后用白藜芦醇处理均能使成纤维细胞存活率提高、SOD活性增强、MDA产生减少。结论低浓度白藜芦醇可促进成纤维细胞增殖，从而对因UVB照射而损伤的体外培养成纤维细胞起到保护作用。

Objective To explore the protective effeet of resveratrol against oxidative damage to cultivated fibroblasts irradiated with UVB. Methods Fibroblasts from normal human skin eultured in vitro were divided into 5 groups （a normal control group, a group irradiated with UVB, a group treated with resveratrol before UVB irradia- tion, and a group treated after irradiation）. A monolayer of fibrohlasts was irradiated with UVB at 60 mJ/cm2. The vitality of the cells was measured using the methylthiazol tetrazolium （MTT） method. The activity of superoxide dismutase （SOD） and malondialdehyde （MDA） content were determined using enzyme biochemistry. Results Res- veratrol over 100μM inhibited the proliferation of fibroblasts. Resveratrol under 100 μM improved the proliferation of cells. The optimal concentration was 50μM. UVB irradiation decreased the vitality of the cells and SOD activity, and it significantly enhanced MDA content. Conclusions Resveratrol treatment before or after UVB irradiation elevates the survival rate of fibroblasts, enhances the activity of SOD, and decreases MDA content. Resveratrol at low concen- tration could improve the proliferation of fibroblasts, and at high concentration could inhibit their proliferation. Resveratol at 50μM relieves the inhibited proliferation of fibroblasts damaged by UVB irradiation.