轻度认知功能障碍及老年性痴呆患者外周淋巴细胞糖原合成酶激酶-3的变化

Glycogen synthase kinase-3 in the lymphocytes of patients with mild cognitive impairment and Alzheimer＇s disease

目的探讨轻度认知功能障碍（MCI）及阿尔茨海默病（AD）患者外周淋巴细胞糖原合成酶激酶-3（GSK-3）的变化。方法用放射性配体结合试验检测正常老年人（对照组）、MCI患者（MCI组）及AD患者（AD组）外周淋巴细胞GSK-3的活性，并用Westernblot法检测GSK-3pSet9位点的磷酸化水平及总GSK-3B表达。结果AD组及MCI组GSK-3活性分别为（1．32±0．09）和（1．22±0．10），明显高于对照组的（1．00±0．06），差异有统计学意义（均P〈0．01）；AD组及MCI组GSK-3BSer9位点的磷酸化水平分别为（0．82±0．07）和（0．87±0．08），明显低于对照组的（1．01±0．07），差异有统计学意义（P〈0．05或0．01）；AD组及MCI组总GSK-3p表达水平与对照组比较，差异无统计学意义（P〉0．05）。结论MCI及AD患者外周淋巴细胞GSK-3的活性升高，可能对此类疾病的早期诊断有潜在的价值。

Objective To explore changes in glycogen synthase kinase-3 （GSK-3） levels in the lympho- cytes of patients with mild cognition impairment （MCI） and Alzheimer＇s disease （AD）. Methods GSK-3 activity was measured by 3219 liquid scintillography in a control group, an MCI group and an AD group. The expressions of GSK-313at Ser9 and total GSK-313 were determined by western blotting. Results GSK-3 levels were significantly higher in the AD and MCI groups than in the control group. The phosphorylation of GSK-313at Ser9 was significantly lower in the AD and MCI groups compared with the control group, but there was no difference in the phosphorylation of total GSK-313 among the three groups. Conclusions Higher GSK-3 activity in the lymphoeytes might have value in the diagnosis of MCI and early AD.