安吉白茶多糖的微波辅助提取及其抗氧化活性

Study on Microwave-assisted Extraction Technology of Anji White Tea Polysaccharides and Its Anti-oxidant Activity

采用微波辅助法从安吉白茶中提取多糖,用硫酸-苯酚法显色,在540nm处运用分光光度计测其吸光度值,计算多糖提取率;考察微波功率,浸提温度,微波处理时间,浸提时间对多糖提取率的影响,并以正交试验设计优化工艺条件,结果表明:影响安吉白茶多糖提取率因素的主次关系是:微波功率>微波处理时间>浸提温度>浸提时间。正交试验最佳条件:微波功率240W、微波时间4min、浸提温度65℃、浸提时间3h,此条件下的白茶多糖得率为16.23%。安吉白茶多糖能够明显的在体外抑制人血红细胞自氧化和H2O2所致红细胞氧化溶血作用,随着茶多糖浓度的增加抑制红细胞的自氧化溶血作用也逐渐增强。当茶多糖浓度为7mg/mL,其溶血度为13.691%与相同浓度的Vc溶液相当,当浓度<7mg/mL时,茶多糖对红细胞溶血的抑制作用比相同浓度下Vc作用弱。7mg/mL的茶多糖对H2O2对所致红细胞的溶血度为22.781%与3mg/mL的Vc溶液作用相当,相同浓度下的茶多糖抗氧化活性比Vc溶液弱。

Microwave- assisted extraction is adopted to extract polysaccharide from Anji White Tea . sulphuric acidphenol method was used for coloration, and the content of polysaccharide was determined by the spectrophotometer at 540 rim. The effects of Microwave intensity, temperature of extraction, Microwave-treating time and the influence of Microwave-treating time were studied. It was found that the order of elements influencing the extraction was： KV of Microwave 〉 Microwave-treating time 〉 temperature of extraction 〉 time of extraction. The optimized condition for the extraction was： KV 240 W, Microwave-treating time： 4 min; extracting at 65℃ for 3 h. Under the above conditions the extracting yield of polysaccharide was 16.23%. Anji White Tea polysaccharide exhibits a significant potency in inhibiting the hemolysis of red blood cells itself and H2O2 induced hemolysis. With the increase of polysaccharide＇ s concentration, the inhibition of auto-oxidation of red blood cell hemolysis increased gradually. When the concentration was up to 7mg/mL, the hemolysis was 13. 691% which equals to Vc with same Concentration. When the TPs was less than 7 mg/mL, its inhibition of hemolysis of red blood cells was weaker than Vc in same concentration. The inhibition of hemolysis due to H202 by 7 mg/mL TPs was 22. 781% which equals to 3mg/mL of V.. The antioxidant of TPs was not as good as the same concentration of Vc solution.