八仙草化学成分研究

Chemical constituents of Galium aparine

目的对中草药八仙草（Galium aparineL.）全草的60%乙醇提取物进行化学成分研究。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及制备薄层等方法进行分离纯化,通过波谱解析和理化鉴别进行结构鉴定。结果分离鉴定了11个化合物,分别为对羟基苯甲酸（1）,香草酸（2）,原儿茶酸（3）,胞嘧啶（4）,胞嘧啶核苷（5）,尿嘧啶（6）,尿嘧啶核苷（7）,腺嘌呤核苷（8）,1,3-二羟基蒽醌（9）,1,3,6-三羟基-2-甲基蒽醌-3-O-新橙皮糖苷（10）,1,4-二羟基-3-异戊烯基-2-萘酸甲酯双葡萄糖苷（11）。结论化合物1-11均为首次从该植物中分离得到。

Objective To investigate the chemical constituents of 60% alcohol extract of Galium aparine L.Methods The compounds were isolated by column chromatography over silica gel and Sephadex LH-20 coupled with preparative silica gel TLC.Structures of the compounds were elucidated on the basis of physicochemical properties and spectral data.Results Eleven compounds were isolated and identified as 4-hydroxybenzoic acid（1）,vanillic acid（2）,protocatechuic acid（3）,cytosine（4）,cytidine（5）,uracil（6）,uridine（7）,adenosine（8）,xanthopurpurin（9）,2-methyl-1,3,6-trihydroxy-9,10-anthraquinone-3-O-α-rhamnosyl（1→2）-β-D-glucoside（10）,2-carbomethoxy-3-prenyl-1,4-naphthohydroquinone-di-O-β-D-glucoside（11）,respectively.Conclusion Compounds 1-11 are isolated from this plant for the first time.