脂肪酶AK对富勒烯封端聚己内酯的降解

Degradation of a fullerene end-capped polycaprolactone by lipase AK

采用滴定法并以聚己内酯PCL为对照,探讨了富勒烯封端聚己内酯C60-PCL的脂肪酶AK降解行为.实验发现,PCL和C60-PCL的酶解反应均符合米氏方程,其动力学参数Km分别为0.75和0.16mg·mL·1,最大降解速度Vmax分别为0.90和0.43mL.h·1.在同等条件下C60-PCL降解速率较PCL减小,表明富勒烯的引入使聚己内酯难于被脂肪酶降解.

Using titrimetric method,enzymatic behavior of lipase AK against fullerene end-capped polycaprolactone （C60-PCL） was studied for the first time,compared with polycaprolactone （PCL）. The results showed that degradation of both C60-PCL and PCL by lipase followed Mechaelis-Menten equation,with the degradation parameters of PCL and C60-PCL were 0.75 and 0.16 mg·mL^-1 for Km,and 0.90 and 0.43 mL·h^-1 for Vmax,respectively. C60-PCL had a slower degradation rate than PCL under the same conditions. These data indicated that the introduction of fullerene enabled PCL hard to be degraded by lipase.