不同产地黄芪中黄芪皂苷Ⅰ、黄芪皂苷Ⅱ和黄芪皂苷Ⅳ含量测定

Determination of astragaloside Ⅰ,astragaloside Ⅱ and astragaloside Ⅳ in Radix Astragali from various habitats

目的：研究采用HPLC-ELSD法测定不同产地黄芪药材中的黄芪皂苷Ⅰ、黄芪皂苷Ⅱ和黄芪皂苷Ⅳ的含量。方法：色谱柱为ZORBAX Eclipse XDB-C18（250 mm×4.6 mm,5μm）,流动相为乙腈和水,梯度洗脱,流速1 mL·min^-1,柱温35℃;蒸发光散射检测器,ELSD参数：喷雾器温度为39℃,漂移管温度55℃,载气压力172.4 kPa,增益值为200。结果：黄芪皂苷Ⅰ、黄芪皂苷Ⅱ和黄芪皂苷Ⅳ的线性范围分别为0.8312～8.312μg（r=0.9996）,2.544～16.536μg（r=0.9990）,0.328～3.28μg（r=0.9992）;平均回收率分别为99.1%,98.0%,98.6%,RSD分别为3.9%,2.8%,2.8%。结论：该方法简单快捷,适合于黄芪药材中黄芪皂苷的含量测定研究,为寻找更佳黄芪产地提供了依据。

Objective：To establish an HPLC-ELSD method for the determination of the content of astragaloside Ⅰ,astragaloside Ⅱ and astragaloside Ⅳ in Radix Astragali from various habitats.Methods：The conditions of chromatography：ZORBAX Eclipse XDB-C18（250 mm×4.6 mm,5 μm）was used with a mobile phase of CH3CN-H2O,flow rate：1 mL·min^-1,column temperature：35 ℃,：spray temperature 39 ℃,drift tube temperature：55 ℃,air pressure：172.4 kPa,gain：200.Results：The calibration curves of astragaloside Ⅰ,astragaloside Ⅱ and astragaloside Ⅳ were in good linearity over the ranges of 0.8312–8.312 μg（r=0.9996）,2.544–16.536 μg（r=0.9990） and 0.328–3.28 μg（r=0.9992） respectively;The average recoveries were 99.1%,98.0% and 98.6% with RSD of 3.9%,2.8% and 2.8%,respectively.Conclusion：The method is simple,rapid,sensitive and reproducible for determination of astragalosides in Radix Astragali.and can be used a basis for finding better habitat of Radix Astragali.