姜黄素对缺血/再灌注大鼠神经细胞凋亡的影响

The effect of curcumin on cerebral ischemia/reperfusion induced apoptosis

目的:观察姜黄素对大鼠局灶脑缺血/再灌注后神经细胞凋亡的作用及其机制。方法:采用SD大鼠线栓模型,动物随机分为3组:假手术组(sham组)、缺血/再灌注组(vehicle组)、姜黄素100 mg.kg-1组(Cur 100组),TUNEL法检测皮层神经细胞凋亡;Western-blot检测凋亡诱导因子(AIF)和细胞色素C(Cytc)在缺血皮层的表达;同时检测膜电位的变化。结果:I/R组凋亡细胞多于sham组及Cur 100组,Cur 100能够显著改善I/R所引起的线粒体膜电位下降;降低AIF,Cytc的表达。结论:姜黄素能够显著减少缺血性神经细胞凋亡的发生,可能与保护线粒体功能,减少AIF和Cytc的释放有关。

Objective：To investigate the effect of curcumin on middle cerebral artery occlusion rats and its mechanism.Method：Cerebral ischemia reperfusion（IR）injury were used induce MCAO in rats.Administration of curcumin 100 mg·kg^-1,i.p.30 min.after MCAO,sham group and vehicle group were treated with equal vehicle respectively.24 h after MCAO,cortex was harvested to analyze apoptosis with Tunnel staining.Also,Western-blot was used to study the expression of AIF and Cytc proteins.Meanwhile,mitochondrial membrane potential was assayed.Results：Curcumin significantly attenuated apoptosis induced by MCAO.AIF and Cytc protein expression were reduced by curcumin.Also,the increased mitochondrial membrane potential was restored by curcumin.Conclusion：Curcumin can significantly attenuated apoptosis through reduction of AIF and Cytc released from mitochondria.