超声击破造影剂微泡阻断正常肝血流灌注的造影观察

Ultrasound cavitation blockage of hepatic circulation analyzed by contrast perfusion imaging

目的 采用视觉评分方法分析微泡增强的超声空化阻断兔正常肝血流灌注后的恢复情况.方法 健康新西兰兔24只,分为超声微泡组、单纯超声组及假照组.超声微泡组经兔耳缘静脉推注脂质微泡联合超声辐照兔肝,单纯超声组以生理盐水代替微泡,假照组超声假照.各组治疗前及治疗后0 min、15 min、30 min、45 min、60 min和24 h对兔肝进行超声造影,视觉评分分析各时间点造影灌注峰值灰阶变化.结果 各组治疗前肝血流灌注比较,视觉评分差异无统计学意义（P〉0.05）;超声微泡组的治疗前造影血流灌注评分显著优于治疗后0 min、15 min两个时间点,差异有统计学意义（P〈0.05）;但与治疗后30 min、45 min、60 min、24 h视觉评分差异无统计学意义（P〉0.05）;而单纯超声组、假照组的治疗前后各时间点之间超声造影评分差异均无统计学意义（P〉0.05）.结论 微泡增强的超声空化具有显著的暂时性阻断兔肝实质血流作用.

Objective To analyze the normal hepatic contrast perfusion blocked by ultrasound excited microbubble cavitation using the visual scoring method. Methods Twenty-four healthy New Zealand rabbits were divided into the microbubbles group （MB ＋ US）, the simple ultrasound group （US） and the sham group. The MB ＋ US group was insonated by US and intravenous injection of lipid microbubbles. Microbubble was replaced by saline in the US group and sham US exposure was used in the sham group. US contrast liver perfusion imaging was performed before and 0 min,15 min,30 min,45 min,60 min,24 h after treatment in each group. Results The visual perfusion scores of each group before treatment were no statistical difference （ P 〉0. 05）. The visual score of pre-treatment significantly higher than that of post 0 min, 15 min in the MB＋ US group （ P〈0. 05）, but no difference with post 30 min,45 min,60 min and 24 h （ P 〉0. 05）. There were no statistical significance between all the time points of the US and the sham groups. Conclusions Ultrasound excited microbubble cavitation can temporarily and significantly interrupt liver blood perfusion in the visual score analysis.