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Derivation of embryonic stem cells from Brown Norway rats blastocysts 被引量：5

Derivation of embryonic stem cells from Brown Norway rats blastocysts
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摘要 Knockout Brown Norway （BN） rat could be a useful disease model for human disorders,however,a failure to derive embryonic stem （ES） cells disturbs the further development of the model.In this study,we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley （SD） rats.The BN rat ES cells expressed the key transcription factors,and were able to form embryonic bodies （EBs） when being differentiated in vitro.After injecting the BN rat ES cells into the SD rat blastocysts,high-contribution chimeric rats were generated and could survive to their adulthood.Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future. Knockout Brown Norway （BN） rat could be a useful disease model for human disorders,however,a failure to derive embryonic stem （ES） cells disturbs the further development of the model.In this study,we reported a case of successful derivation of the BN rat ES cells with the derivation efficiency comparable to that of Sprague Dawley （SD） rats.The BN rat ES cells expressed the key transcription factors,and were able to form embryonic bodies （EBs） when being differentiated in vitro.After injecting the BN rat ES cells into the SD rat blastocysts,high-contribution chimeric rats were generated and could survive to their adulthood.Our success in generating pluripotent rat ES cells will benefit the generation of the knockout rats in the future.
作者 Xiaoyang Zhao Zhuo Lv Lei Liu Liu Wang Man Tong Qi Zhou
机构地区 State Key Laboratory of Reproductive Biology Graduate School of Chinese Academy of Sciences
出处 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2010年第7期467-473,共7页 遗传学报（英文版）
基金 supported in part by the grants from the Chinese National Basic Research Program (No 2007CB948001) the National High Technology R&D Program of China (No 2006AA02A101) Integrated Project funded by the Sixth Framework Programme (FP6) of the European Union (Contract:LSHG-CT-2005-019015)
关键词 Brown Norway （BN） rat embryonic stem cells PLURIPOTENCY chimera rat Brown Norway （BN） rat embryonic stem cells pluripotency chimera rat
分类号 Q813 [生物学—生物工程] Q95-331 [生物学—动物学]
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参考文献18

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Journal of Genetics and Genomics
2010年 第7期

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