非小细胞肺癌患者外周血CD4^+CD25^+Foxp3^+调节性T细胞检测及临床意义

Peripheral CD4^+CD25^+Foxp3^+ Regulatory T cells in Patients with NSCLC and Its Clinical Significance

[目的]探讨非小细胞肺癌患者外周血CD4+CD25+Foxp3+调节性T细胞(Treg)表达及临床意义。[方法]流式细胞术检测30例非小细胞肺癌组患者及12例健康志愿者外周血CD4+CD25+Foxp3+Treg百分比。[结果]肺癌组外周血CD4+CD25+Foxp3+细胞百分比(6.24%±2.01%)高于健康对照组(4.83%±0.85%)(P<0.05),外周血CD4+CD25+Foxp3+细胞百分比在肺癌患者不同性别、年龄、病理、KPS及肿瘤标志物正常与否之间比较无差异(P>0.05),Ⅳ期患者表达高于Ⅰ～Ⅲ期患者(P<0.05),带瘤患者表达高于无瘤患者(P<0.05),有气虚证、血瘀证患者高于无气虚证、血瘀证患者(P<0.05)。[结论]检测肺癌患者外周血CD4+CD25+Foxp3+Treg表达在了解患者肿瘤进展情况和机体免疫功能状态方面有一定价值,其表达升高与患者出现气虚证和血瘀证有关。

[Purpose] To investigate the expression of CD4＋CD25＋Foxp3＋ regulatory T cells（Treg）in peripheral blood in patients with NSCLC and its significance.[Methods] The proportion of CD4＋CD25＋Foxp3＋ Treg in thirty cases with NSCLC and 12 healthy controls were measured by flow cytometry.[Results] The proportion of CD4＋CD25＋Foxp3＋ Treg in peripheral blood from patients with NSCLC（6.24%±2.01%） was higher than that from healthy controls（4.83%±0.85%）（P0.05）.The proportion of peripheral CD4＋CD25＋Foxp3＋ Treg in patients with NSCLC was not different with agender,age,pathology,KPS score and tumor marker level（P0.05）.The proportion of CD4＋CD25＋Foxp3＋ Treg in the patients with NSCLC stage Ⅳ was higher than that in stage Ⅰ～Ⅲ（P0.05）.The proportion of CD4＋CD25＋Foxp3＋ Treg in the patients bearing tumor was higher than those without tumor（P0.05）.The proportion of CD4＋CD25＋Foxp3＋ Treg in patients with Qi deficiency syndrome or blood stasis syndrome was higher than those without the syndromes（P0.05）.[Conclusion] Detecting the expression of CD4＋CD25＋Foxp3＋ Treg in peripheral blood of patients with NSCLC is helpful for knowing cancer progression and the patients immunity.The increase of the proportion of CD4＋CD25＋Foxp3＋ Treg closely correlates to Qi deficiency syndrome and blood stasis syndrome.