高压氧对脑损伤大鼠行为学的影响

Effect of hyperbaric oxygen on changes in behavior after brain damage in rats

目的 研究高压氧（hyperbaric oxygen,HBO）治疗对脑损伤大鼠行为学的影响,为临床HBO治疗颅脑损伤提供实验依据.方法 Wistar雄性大鼠21只,随机分成HBO治疗组、脑损伤组及正常对照组,每组7只.应用液压撞击仪制作侧位液压冲击脑损伤大鼠模型,HBO组于术后24 h开始行HBO治疗,1次/d,连续10 d.各组从术后第6天开始应用Morris水迷宫实验测试并比较大鼠的行为能力、学习和记忆能力.结果 定位航行实验：前5 d的逃避潜伏期正常对照组〈HBO组〈脑创伤组（P〈0.05）;空间探索试验：穿越平台的次数正常对照组〉HBO组〉脑创伤组,各组比较差异均有统计学意义（P〈0.05）.结论 HBO治疗对脑损伤大鼠学习、记忆能力的恢复有促进作用.

Objective To investigate effect of hyperbaric oxygen on changes in behavior after brain damage in rats and to find out laboratory evidence for clinical application of HBO in the treatment of brain damage. Methods Twenty-one male Wister rats were randomly divided into 3 groups： the HBO group, the control group and the brain damage group, each consisting of 7 animals. The lateral hydraulic impact instrument was used to develop the rat model of impact brain damage. The HBO group was given HBO treatment 24 hours after injury, for a succession of 10 days. Six days after impact damage, each group was measured respectively with Morris water maze to compare ethological abilities of learning and memory between the groups. Results The positioning navigation test （PNT） obtained the following results： for the first 5 days, the control group was shorter in evasion latency than the HBO group, and the HBO group was shorter than the brain damage group（P〈0.05）. Spatial probe test （SPT） indicated that the control group was 〉 the HBO group 〉 the brain damage group for the number of passing across the platform （P 〈 0. 05）. Conclusions HBO therapy could enhance the abilities of brain damaged rats in learning and memory.