高压氧对心肌梗死大鼠模型梗死面积及左室功能的影响

Effect of hyperbaric oxygen treatment on infarct size of acute myocardial infarction and on the function of left ventricle in rats

目的 探讨高压氧（HBO）治疗对急性心肌梗死（AMI）大鼠模型梗死面积及左室功能的影响.方法 50只健康雄性SD大白鼠,分为左冠状动脉未结扎组12只,另38只以手术方式结扎左冠状动脉,术后6 h 5只大鼠死亡,存活的33只再分为HBO对照组（n=16）和HBO组（n=17）,HBO组心肌梗死后应用HBO治疗,7 d后测量心肌梗死面积、左心室收缩压和舒张压差（LVEDP）及左心室内压最大上升和下降速率（±dp/dtmax）的变化.结果 HBO组与HBO对照组相比,心肌梗死面积明显减小（P〈0.05）,左室功能明显好转（P〈0.05）.结论 及早应用HBO治疗可以明显减少AMI大鼠模型心肌坏死面积,并且可以改善左室的收缩及舒张功能.

Objective To investigate effect of hyperbaric oxygen treatment on infarct size of acute myocardial infarction and on the function of left ventricle in rats. Methods An acute myocardial infarction model was developed by using 50 male SD rats, which were randomly divided into 3 groups： the sham group（12 rats）, the acute myocardial infarction group （AMI） （16 rats） and the HBO group（17 rats）. Hyperbaric oxygen treatment was applied to the HBO group for a succession of 7 days. Infarct sizes, differences between systolic and diastolic pressures of the three groups and changes in cardiac hemodynamics （± dp/dtmax） were measured 7 days after treatment. Results When compared with that of the AMI group, infarct size and left ventricle function of the HBO group improved significantly （P 〈 0. 05）. Conclusions Early administration of HBO could significantly reduce infarct size and improve left ventricle function of rats with AMI.