尾加压素Ⅱ及其受体在人肾透明细胞癌组织中的表达及意义

Expression of Urotensin Ⅱ and its Receptor in Human Renal Clear Cell Carcinoma Tissues

目的:测定肾透明细胞癌中尾加压素Ⅱ(UⅡ)与其受体(UT-R)的表达量,以期探讨UⅡ与UT-R的表达在肾透明细胞癌发生中的临床意义。方法:对12例肾透明细胞癌肿瘤组织通过免疫组化检测肾透明细胞癌组织和正常肾组织UⅡ蛋白的表达,并采用逆转录-多聚酶链式反应(RT PCR)的方法测定肾透明细胞癌组织和正常肾组织UⅡmRNA和UT-RmRNA的表达量。结果:肾透明细胞癌组织和正常肾组织中有UⅡ蛋白表达。RT-PCR结果显示肾透明细胞癌中UⅡmRNA的表达明显高于正常肾组织0.92±0.09和0.62±0.06(P<0.01)。UT-R mRNA的表达明显高于正常肾组织0.28±0.01和0.16±0.03(P<0.01)。结论:肾透明细胞癌中UⅡ和UT-RmRNA高表达提示UⅡ/UT系统可能与肾透明细胞癌的发生、发展有关,UⅡ可能通过自分泌或旁分泌的方式发挥作用。

Objective：To examine the expression of U Ⅱ and UT-R in human renal clear cell carcinoma tissues and attached non neoplastic renal tissues in order to assess a possible autocrine/paracrine role of U Ⅱ in renal tumors. Methods： Twelve renal clear cell carcinoma tissues and normal renal tissues were obtained from patients received radical nephrectomy. U Ⅱ protein was measured by immunohistochemistry, and U Ⅱ mRNA and UT-R mRNA expression was analyzed by RT-PCR. Results：Renal clear cell carcinoma and normal kidney protein expression in U Ⅱ. RT - PCR results showed that renal cell carcinoma U Ⅱ mRNA was significantly higher than normal kidney tissue0. 92 ± 0.09 and0. 62 ± 0.06 （ P d0.01）. UT- R mRNA expression was significantly higher than normal kidney tissue 0 . 28 ± 0 . 01 and 0 . 16 ±0 . 03 （ P 〈0 . 01）. Conelusions..The overexpression of U Ⅱ and UT RmRNA in renal clear cell carcinoma of U Ⅱ and UT RmRNA overexpression of U Ⅱ/UT system may indicate UⅡ / UT system is associated with renal clear cell carcinoma, UⅡ may play a role through the autocrine or paracrine manner. Key words