NaCl和壳聚糖对百里香不定芽增殖及其生理指标的影响

Propagation and Physiology Indexes of Adventitious Buds of Thymus vulgaris Treated by NaCl and Chitosan

以离体培养的百里香不定芽为材料,研究了添加不同浓度NaCl和壳聚糖对不定芽增殖及生理指标的影响.结果显示:添加0.25～1.0g?L-1NaCl和0.1～0.2g?L-1壳聚糖都能不同程度促进不定芽的增殖,其中以添加0.5g?L-1LNaCl和0.2g?L-1壳聚糖的增殖效果最佳,增值系数比对照分别显著增加39.78%和45.01%.0.5g?L-1NaCl和0.2g?L-1壳聚糖处理的不定芽增殖过程中,其可溶性蛋白含量先上升后下降,其峰值分别比对照增加38.31、22.78μg?g-1,而可溶性糖含量则先下降后上升,其峰值分别比对照高0.16、1.29mg?g-1;不定芽增殖过程中SOD活性呈先上升后下降的变化趋势,而POD活性的变化为先下降后上升,CAT活性则一直呈缓慢下降的趋势,但它们的活性较对照均有所提高.研究表明,添加0.5g?L-1NaCl和0.2g?L-1壳聚糖都能显著提高不定芽的增殖能力,并能很好地调节自身营养物质含量和保护酶系统活性,维持不定芽正常的生理代谢功能,使之表现出更强的增殖能力.

Thymus vulgaris adventitious buds in vitro were used to study the effect of NaCl and chitosan in propagation of T.vulgaris.The results showed that adding 0.25～1.0 g·L-1 NaCl and 0.1～0.2 g·L-1 chitosan concentration could help adventitious buds proliferation,and the best proliferation was found when adding 0.5 g·L-1 NaCl and 0.2 g·L-1 chitosan.Compared with the control,proliferation coefficient increased 39.78% and 45.01% adding NaCl or chitosan,respectively.During the process of proliferation in 0.5 g·L-1 NaCl and 0.2 g·L-1 chitosan concentration,the content of soluble protein content increased and then decreased,its peak value were increased 38.31 and 22.78 μg·g-1,respectively.While the soluble sugar content is first decreased and then increased,the peak higher 0.16 and 1.29 mg·g-1,respectively than that of the control;SOD activity of adventitious buds first increased and then decreased,while in contrary for the POD activity.CAT activity was decreased slowly with the time.Our studies showed that 0.5 g·L-1 NaCl and 0.2 g·L-1 chitosan can enhance the proliferation of adventitious buds.The differences were significant compared with control.Both 0.5 g·L-1 NaCl and 0.2 g·L-1 chitosan can enhance their nutritive material contents and the activities of protective enzyme in adventitious buds.As the results,the adventitious buds maintain the normal physiological metabolism show strong proliferation.