5-羟色胺免疫电极的研制和应用

DEVELOPMENT AND APPLICATION OF 5 HT IMMUNOELECTRODE

研制了电位型无标记５－羟色胺（５－ＨＴ）免疫电极。以偶联法制备抗体膜，结合抗原后的复合膜与空白膜的△Ｅ值与５－ＨＴ的浓度成线性关系。电极的线性范围为１．１８×１０－７～６．２７×１０－６ｍｏｌ／Ｌ，检测下限为１．１２×１０－７ｍｏｌ／Ｌ，斜率为－１３．０８ｍｌ，相关系数ｒ＝－０．９９８４，对血中其他物质选择性良好，抗体膜片一次性使用，以标准曲线法测定了牛血小板中５－ＨＴ浓度。

The preparation and application of a potential unlabeled 5 HT immunoelectrode was reported The antibody membrane was prepared in coupling method. The difference △E between antibody antigen complex membrane and blank membrane had a linear relationship with 5 HT concentration. Using the immunoelectrode the linear range for 5 HT was 1.18×10 -7 ～6.17×10 -6 mol/L, and the limit of detection was 1.12×10 -7 mol/L. The slope was -13.08mV/p 5 HT and coefficient of correlation was -0.9974. The selectivity for some substances in blood was good. The antibody membrane was disposable. The 5 HT contents of bull platelet sample were measured by standard curve method.