RP-HPLC法同时测定复方氧氟沙星滴鼻液中两组分的含量

SIMULTANEOUS DETERMINATION OF OFLOXACIN AND DEXAMETHASONE SODIUM PHOSPHATE IN COMPOUND OFLOXACIN NASAL DROPS BY RP HPLC

ＲＰ－ＨＰＬＣ法，在ＳｈｉｍｐａｃｋＣＬＣ－ＯＤＳ柱上同时测定复方氧氟沙星滴鼻液中氧氟沙星和地塞米松磷酸钠的含量。平均回收率氧氟沙星为９９．８±０．５％，地塞米松磷酸钠为１００．６±０．８％（ｎ＝５）。

An RP HPLC method was developed to dertermine the ofloxacin and dexamethasone sodium phosphate in a compound ofloxacin nasal drops. The chromatographic conditions comprised Shimpack CLC ODS column (6 0mm×150mm, 5μm), a mixture of methanol citric acid solution (0 05mol/L) acetoni trile ammonium acetate solution(0.5mol/L) 10g/L phosphoric acid solution (100∶75∶22∶1∶2) as mobile phase, the flow rate being 1.0ml/min, and the detection wavelength being 242nm. The average recoveries of ofloxacin and dexamethasone sodium phosphate were 99.8±0.5% ( n=5 ) and 100.6±0 8% ( n=5) respectively. This method is suitable for quality control of the preparation.