不同压力CO2气腹对胃癌细胞裸鼠移植瘤成瘤能力的影响

Effects of CO2 pneumoperitoneum pressures on growth and proliferation of gastric cancer cells in nude mice transplanted tumor

目的 通过将不同压力CO2气腹环境下培养的胃癌MKN-45细胞接种于裸鼠皮下,观察CO2气腹对胃癌细胞体内生长增殖的影响.方法 将胃癌MKN-45细胞置于0、10、12和15 mm Hg CO2气腹环境下培养4 h,然后取2×106个细胞接种于裸鼠皮下,3周后处死裸鼠,测量肿瘤的体积和重量,再对各组移植瘤行HE染色和Ki-67免疫组化染色.结果 0、10、12 mm Hg CO2气腹组裸鼠移植瘤成瘤时间、肿瘤体积和重量（分别为7.8 d、7.2 d、7.8 d 1.2 cm3、1.3 cm3、1.3 cm3 1.5 g、1.9 g、1.6 g）与对照组（分别为7.3 d、1.2 cm3、1.4 g）比较差异均无统计学意义（P〉0.05）,15mm Hg CO2气腹组移植瘤成瘤时间（12.5 d）与对照组比较显著延长,肿瘤体积（0.5 cm3）和重量（0.5 g）与对照组比较显著下降（P〈0.05）.0、10、12 mm Hg CO2气腹组裸鼠移植瘤细胞内Ki-67抗原阳性率（61.2%、60.5%、63.4%）与对照组（59.6%）比较差异均无统计学意义（P〉0.05）,15 mm Hg CO2气腹组的阳性率（27.5%）与对照组比较显著下降（P〈0.01）.结论 临床常用压力CO2气腹对胃癌MKN-45细胞体内生长增殖无显著影响.

Objective To investigate the effects of different CO2 pneumoperitoneum pressures on gastric cancer cells＇ growth and proliferation in nude mouse model of implanted tumor. Methods Human gastric cancer cell lines MNK-45 were exposed under 0、10、12 and 15 mm Hg CO2 pneumoperitoneum for 4 hrs respectively. 2 × 106 processed cells were inplanted into nude mice subcutaneously. Three weeks later, mice were sacrificed and the weight and bulk of the tumor measured. Then we observed the transplantation tumor by HE stain and Ki-67 stain. Results There was no significant difference in tumor＇s growing time, bulk and weight between 0, 10, 12 mm Hg CO2 pneumoperitoneum groups （7. 8 d, 7. 2 d, 7. 8 d 1. 2 cm3, 1. 3 cm3, 1. 3 cm3 1.5 g, 1. 9 g, 1. 6 g）and the control group （7. 3 d, 1. 2 cm3, 1.4 g） （P 〉 0. 05 ）. The growing time of tumor in 15 mm Hg CO2 pneumoperitoneum （12. 5 d） was obviously longer than the control group （ P 〈 0.05 ） , the bulk and weight of tumor in 15 mm Hg CO2 pneumoperitoneum （0. 5 cm3, 0. 5 g） group significantly decreased compared with the control group （P 〈0.05）. The positive rate of Ki-67 in 15 mm Hg CO2 pneumoperitoneum （27. 5% ） group was obviously lower than the control group （59.6%） （P〈0.01）. However, there were no significant differences between 0, 10, 12 mm Hg CO2 pneumoperitoneum groups （61.2%, 60.5%, 63.4%） and the control group （P 〉 0.05）. Conclusion Clinically adopted CO2 pneumoperitoneum pressures have no significant effect on gastric cancer cells growth and proliferation.