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九例成人T淋巴细胞白血病的临床与实验研究 被引量:4

Clinical and experimental study of 9 cases of adult T cell leukemia
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摘要 目的探讨成人T淋巴细胞白血病(ATL)的临床、细胞学、免疫学、细胞遗传学、血清学及分子生物学等特征。方法采用间接免疫荧光法及酶联免疫吸附法检测血清HTLVⅠ抗体,建立PCR和液相杂交法检测前病毒基因序列,结合MIC特征确诊ATL。结果和结论确诊9例ATL,其临床特征主要为淋巴结肿大,皮损及溶骨改变少见,外周血及骨髓出现典型的花瓣状核淋巴细胞,胞体大,核畸形,免疫表型为CD-1、CD+2、CD3+、CD4+、CD-8,还表达CD25等激活标记,染色体核型及HLA分型未发现有规律性的变化。8例ATL中有7例血清HTLVⅠ抗体阳性,应用PCR及液相杂交法可检出整合到宿主细胞DNA中的HTLVⅠ病毒序列,表明在我国HTLVⅠ也是ATL的病因。9例中1例为淋巴瘤型,1例为慢性型,7例为急性型ATL。 Objective In order to clarify the clinical and laboratory features of adult T cell leukemia (ATL): morphology, immunology, cytogenetics, serology and molecular biology. Methods Indirect immunofluorescence assay and ELISA were used to detect serum HTLV Ⅰ antibody. The HTLV Ⅰ provirus sequence were amplified by PCR and confirmed by liquid hybridization. Results and Conclusion Nine cases of ATL were diagnosed. The major clinical manifestation was lymph node enlargement found in all patients. Skin involvement and osteolysis were not frequent. The characteristic finding was leukemic cells with highly indented or lobulated flower like nuclei in peripheral blood and bone marrow. ATL cells were CD 2,CD 3,CD 4,CD 25 positive and CD 8 negative. No specific chromosome abnormality or HLA type was found. Seven of 8 patients examined had HTLV Ⅰ antibody. The HTLV Ⅰ provirus genome sequence integrated into host cell DNA was amplified by PCR and confirmed by liquid hybridization. All of these results showed that HTLV Ⅰ was also the etiological agent of ATL in China. One of the 9 cases of ATL was classified as lymphoma type, one as chronic type, and the rest as acute type.
出处 《中华血液学杂志》 CAS CSCD 北大核心 1999年第2期73-75,共3页 Chinese Journal of Hematology
基金 福建省卫生厅科研基金
关键词 白血病 T细胞白血病 聚合酶链反应 HTLV-I Leukemia Lymphoma,T cell,acute,HTLV Ⅰ associated HTLV Ⅰ Polymerase chain reaction
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  • 1曾毅,中华血液学杂志,1986年,7期,471页
  • 2王志澄,中华血液学杂志,1985年,6期,50页
  • 3胡建达,中华医学杂志,1995年,75卷,424页

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