摘要
目的研究药物诱导白血病细胞凋亡中的电化学变化及其意义。方法使用电化学传感器检测经足叶乙甙(Vp16)诱导的HL60细胞凋亡。结果2~200μg/mlVp16作用于HL60细胞24小时的过程中,随着药物剂量的增大,细胞峰值电流逐渐降低,Vp162μg/ml时即可观察到细胞电化学活性较对照组下降,20μg/ml时明显下降。以Vp16200μg/ml诱导细胞凋亡,通过DNA含量分析显示在2,3,4小时细胞凋亡率为10.5%~20.0%,用电化学传感器方法检测细胞电化学活性改变差异明显,峰值电流从1.8μA降至04μA,4小时时峰值电流降至用药前的1/5。结论在细胞凋亡早期启动阶段的电化学信号改变具有一定的早期意义和蓄积性。
Objective To study the electrochemical behavior of apoptotic HL 60 cells induced with etoposide. Methods Electrochemical cytosensor was used to monitor HL 60 cells treated with etoposide. Results The peak current and electron transfer rate decreased as etoposide dosage increased. The decrease appeared at 2μg/ml etoposide treatment and was remarkable at 20μg/ml, which occurred before the typical changes of cell morphology and DNA contents for apoptosis. FCM DNA analysis showed that the apoptotic cells were of 10.5%~20% while the peak current decreased from 1.8μA to 0.4μA after 2,3 and 4 hours treatment with 200μg/ml etoposide, and the peak current after 4 hours treatment was 5 times lower than that before induction. Conclusion The changes of electrochemical behavior during the early stage of apoptosis occurred earlier and could be accumulated.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1999年第2期82-84,共3页
Chinese Journal of Hematology