摘要
为了解牛分枝杆菌的基因型特点,初步建立适合新疆地区的MIRU-VNTR基因分型方法,本研究利用24个MIRU-VNTR位点,对30株牛分枝杆菌新疆分离株进行基因分型研究。结果显示,在24个MIRU-VNTR位点中,有9个位点(ETR-A、B、D、E;MIRU24;QUB11a、11b、1895、26)具有多态性,可将30株菌分为7个基因型,分型指数为0.611。而国际通用的5个ETRs位点(ETR-A～E)可将该30株菌分为4个基因型,分型指数为0.712。多态性位点中多态性指数较高的5个位点(ETR-B、ETR-D、MIRU24、QUB11a、QUB26)具有与9个位点相似的分型能力,分型指数为0.715。研究表明,新疆地区与其他地区或国家的牛分枝杆菌在基因分型方面存在差异。5个多态性较高的位点均具有良好的分型能力,表明MIRU-VNTR基因分型方法可以作为新疆地区牛分枝杆菌分子流行病学研究的辅助方法。
The genotypic characteristics of Mycobacterium bovis in Xinjiang Uygur Autonomous Region were investigated using MIRU-VNTR genotyping method,the total of 30 M.bovis isolates were gentyped for 24 MIRU-VNTR loci,and 7 different genotypes were identified based on polymotphism at 9 loci（ETR-A,B,D,E;MIRU24;QUB11a,11b,1895,26）,with a genotypic diversity indice of 0.611.The 5 most common ETRs loci resolved the 30 isolates into 4 genotypes,with a genotypic diversity indice of 0.712.The set of 5 most polymorphism loci（ETR-B,ETR-D;MIRU24;QUB11a,QUB26） showed similar discrimination with the 9 polymorphism loci with genotypic diversity indice of 0.715.These results indicated that the M.bovis in Xinjiang Uygur Autonomous Region were different from other regions.The 5 highly polymorphism loci could serve as markers for molecular epidemiology study of M.bovis in Xinjiang Uygur Autonomous Region.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2010年第7期524-528,共5页
Chinese Journal of Preventive Veterinary Medicine
基金
国家863专项基金(2007AA02Z405)
