重庆地区山羊嗜血支原体(附红细胞体)感染率调查及危险性因素评估

Infection rate and risk factors analysis of Haemotrophic Mycoplasma(formerly Eperythrozoon ovis) in Chongqing area

为调查山羊嗜血支原体(附红细胞体)在重庆地区的感染率并评估危害其感染的因素,本研究将采集于重庆14个区县的共1191份山羊血液样本,分别采用染色镜检和PCR方法进行检测,并对不同养殖方式和地理条件进行分析。结果显示:山羊嗜血支原体感染率为16.1%,其中丘陵地区感染率(13.7%)低于山区(18.6%),规模化养殖场感染率(14.3%)低于农户散养(17.1%)。危险性因素分析结果显示:山区山羊嗜血支原体感染率是丘陵地区的1.43倍(OR1.43,95%CI1.05-1.95;χ2=5.13,d.f.=1,p=0.02),并且差异显著;农户散养山羊嗜血支原体感染率是规模化养殖的1.23倍(OR1.23,95%CI0.88-1.71;χ2=1.51,d.f.=1,p=0.22),差异不显著。表明不同的地理条件与山羊嗜血支原体感染率密切相关。

In this study,we collected 1 191 goat blood samples from 14 counties in Chongqing area,of which detected Haemotrophic Mycoplasma（formerly Eperythrozoon ovis） by staining microscopy and 16S rRNA-PCR,and the geographic condition and breed mode were evaluated as risk factors associated with the infection.The results showed that 16.1 % of samples were detected Haemotrophic Mycoplasma positive infection,of which 13.7 % were in hilly areas and 18.6 % in mountain areas.The infection rate of Haemotrophic Mycoplasma in scale farm（14.3 %） was lower than that of unscaled farm（17.1 %）.Infection rate of Haemotrophic Mycoplasma of goats breed in mountain areas was 1.43 times higher than that in hilly areas（OR=1.43,95 % CI=1.05-1.95;χ2=5.13,d.f.=1,p=0.02）,Infection rate in goats with Scatter-Feed mode was 1.23 times higher than that of intensive culture（OR=1.23,95 % CI=0.88-1.71;χ2=1.51,d.f.=1,p=0.22）.The results demonstrated that different geographic condition was highly associated with Haemotrophic Mycoplasma infection of goats.