PPARγ对多器官功能障碍综合征大鼠炎性反应的影响

The effect of PPARγ on the inflammatory response in rats with MODS

目的:观察过氧化物酶增殖物激活受体γ(PPARγ)及其激动剂罗格列酮注对多器官功能障碍综合征(MODS)大鼠炎性反应的影响。方法:50只大鼠随机分为5组:A组(正常对照组),B组(MODS损伤1d组),C组(MODS损伤3d组),D组(PPARγ激动剂预处理1d组),E组(PPARγ激动剂预处理3d组)。观察5组大鼠从致伤开始后1、3d的各脏器功能的生化指标,病理组织学,大鼠外周血单个核细胞内转录因子PPARγ、NF-κBp65的表达及血清TNF-α、IL-6的表达。结果:经股静脉途径注入PPARγ激动剂罗格列酮可降低MODS大鼠总胆红素(TB)、谷丙转氨酶(ALT)、谷草转氨酶(AST)水平;减轻大鼠病理组织学损伤,降低血液中TNF-α、IL-6的水平。结论:通过股静脉途径注入PPARγ激动剂,抑制了NF-κB的活化,阻断其所调控的炎症因子的合成,从而消除炎性细胞在体内的大量聚集,阻断炎症反应的恶性循环,成为治疗MODS新的方向。

Objective：To observe the effect of PPARγ and its agonists-rosiglitazone on the inflammatory response in rat with multiple organ dysfunction syndrome（MODS）.Method：50 rats were randomly divided into five groups：A group（control group）,B group（MODS 1 d）,C group（MODS 3 d）,D group（PPARγ agonist pretreatment 1 d）,E group（PPARγ agonist pretreatment 3 d）.The biochemical indicators and pathology of the organ,the expression of transcription factors PPARγ,NF-κB p65 in mononuclear cells which is in peripheral blood and the expression of TNF-α,IL-6 in blood serum were observed in rats of all five groups at the first day and the third day after injury.Result：Injecting of PPARγ agonists rosiglitazone from the femoral vein can reduce the level of total bilirubin（TB）,alanine aminotransferase（ALT）and aspartate aminotransferase（AST）.Besides,the pathological injury in organs can be alleviated and the level of TNF-α,IL-6 can be reduced in rats with MODS.Conclusion：Injecting of PPARγ agonists from the femoral vein could suppress the activation of the NF-κB and block the synthesis of inflammatory factors which were controlled by NF-κB.Besides,gathering of inflammatory cells in the body could be eliminated and the vicious circle of inflammatory response could be cut out.Injecting of PPARγ agonists may become a new treatment for MODS.