人SSX2IP与14-3-3η蛋白相互作用研究

Preliminary Research on the Interaction between SSX2IP and 14-3-3η

人类SSX2IP是SSX2蛋白的相互作用蛋白,它与肿瘤的发生、发展有着密切的相关性。为进一步阐明人类SSX2IP蛋白的潜在功能,以其作为诱饵蛋白对成人肝cDNA文库进行酵母双杂交筛选,结果筛到了SSX2IP的一个新相互作用蛋白14-3-3η。利用GSTPull-down实验和细胞内免疫共沉淀实验在体内外验证了该相互作用的真实性。通过免疫荧光共定位实验发现SSX2IP和14-3-3η共表达于HeLa细胞时,它们共定位于胞质及核周。利用CCK-8法检测SSX2IP和14-3-3η对HEK293T细胞增殖的影响,发现共转染SSX2IP和14-3-3η后,HEK293T细胞的增殖速度有所提高。这些结果为进一步深入研究SSX2IP与14-3-3η的相互作用及其生物学功能提供新的线索。

SSX2IP（SSX2 interacting protein） was identified as a tumor associated protein in recent studies. The human liver cDNA library was screened with pDBLeu-SSX2IP as bait plasmid by yeast two-hybrid system and 14-3-3η was identified as a SSX2IP interactive protein. The interaction was confirmed by GST Pulldown and coimmunoprecipitation assays. Immunofluorescent localization experiments showed that SSX2IP co-localized with 14-3-3η in the cytoplasm and perinuclear area when co-transformed in HeLa cells. The CCK-8 assay which was employed to detect the effect on cell proliferation when SSX2IP was co-transformed with 14-3-3η in HEK293T cells showed that the proliferation of cells co-transformed with SSX2IP and 14-3-3η was higher than that of both control cells and cells transformed with SSX2IP or 14-3-3η. It seems that to do further research on the interaction of SSX2IP and 14-3-3η may be very important and useful.