RNA干扰NRP2基因表达对胃癌细胞SGC7901裸鼠移植瘤的抑制作用

The Inhibition Effect of RNA Interference Silence NRP2 Expression in Transplantation Tumor of Gastric Carcinoma in Athymic Mouse

目的:探讨特异性抑制NRP2基因表达对人胃癌细胞SGC7901裸鼠移植瘤生长及淋巴管新生的影响。方法:采用小RNA干扰方法,构建shNRP2质粒,稳定转染入SGC7901细胞株,Westen blot检测转染前后NRP2蛋白的表达。建立人胃癌细胞SGC7901裸鼠移植瘤模型,随机分为shNRP2组(实验组)、shCon组(HK阴性对照组)和正常对照组,观察移植瘤的生长情况。6周后处死裸鼠,免疫组化检测NRP2蛋白的表达及微淋巴管密度(Micro-vessel density,MLD)。结果:成功构建shNRP2质粒,与另两组比较,shNRP2组细胞NRP2蛋白表达明显降低,且移植瘤组织生长、NRP2表达及MLD明显受抑制。结论:抑制NRP2基因的表达,可以抑制胃癌裸鼠移植瘤的生长及淋巴管的形成,NRP2基因有可能成为一个潜在的胃癌生物治疗靶点。

Objective：To Investigate the effect on tumor growth and lymphangiogenesis by RNA interference to Specifically inhibit NRP2 gene expression in SGC7901 gastric cancer cells in nude mice.Methods：To construct shNRP2 plasmid by the small RNA interference. To detect NRP2 protein expression before and after transfection by Western blot. To establish the human gastric cancer cell SGC7901 xenografts in nude mice,the modols were randomly divided into shNRP2 group （experimental group）, shCon group（HK negative control group） and normal control group to observe the growth of transplanted tumors.All mice were sacrificed after 6 weeks.The volume and weight of the tumors were measured. The distribution of NRP2 and MLD in the tumors was detected by immunohistochemistry. Results：ShNRP2 plasmid was constructed successfully, compared with the other two groups, NRP2 protein expression decreased significantly in shNRP2 group, and the transplanted tumor tissue growth, NRP2 expression and MLD significantly were inhibited. Conclusion：Inhibition of NRP2 gene expression, can inhibit the growth of gastric cancer xenografts in nude mice and the formation of lymphatic vessels, NRP2 gene may be a potential target for biological therapy of gastric cancer.