添加胰蛋白酶促进Streptomyces hygroscopicus CCTCC M203062 产谷氨酰胺转胺酶？

Enhancement of Microbial Transglutaminase by Streptomyces hygroscopicus CCTCC M203062 Using Tripsin

研究了添加胰蛋白酶对Streptomyces hygroscopicus CCTCC M203062合成谷氨酰胺转胺酶的影响。结果表明,添加胰蛋白酶可以提高发酵过程中谷氨酰胺转胺酶的酶活。摇瓶培养中,在发酵起始时添加200U/ml的胰蛋白酶,谷氨酰胺转胺酶的酶活最高达到了6.61U/ml,比对照提高了27.1%。初步研究表明,添加胰蛋白酶可以直接切割发酵过程中产生的酶原,使其被快速地转化为成熟酶,因此推测胰蛋白酶提高谷氨酰胺转胺酶酶活的原因是解除了酶原的产物抑制作用,产生更多的酶原,从而促进了产酶。

Effects of Tripsin addition on the accumulation of microbial transglutaminase（MTG） by Streptomyces hygroscopicus CCTCC M203062 were investigated. The results showed that the addition of Trypsin enhanced MTG accumulation, and the optimal addition time and concentration of Trypsin were at 0 h and 200 U/ml. The maximum MTG activity in the culture broth was 6.61 U/ml and increased by 27.1% compared with the control. With the addition of Trypsin, pro-TGase was cut to MTG, thus we presume adding Trypsin into the culture broth can enhance the production of pro-TGase by relieving the product inhibition, and the accumulation of MTG was improved.