COPD大鼠模型气道MMP-9和TIMP-1表达的研究

Chronic Obstructive Pulmonary Disease:Expression of Matrix Metalloproteinase-9 and Tissue Inhibitor of Metalloproteinase-1 in Airway of Rat Model

目的:探讨基质金属蛋白酶-9(MMP-9)及组织金属蛋白酶抑制因子-1(TIMP-1)在慢性阻塞性肺疾病(COPD)大鼠模型气道的表达。方法:Wistar大鼠25只随机分为COPD模型组和正常对照组,采用单纯被动吸烟法建立COPD大鼠模型,以酶联免疫吸附法(ELISA)测定两组大鼠血清及支气管肺泡灌洗液(BALF)中MMP-9和TIMP-1的表达水平。结果:COPD组血清及BALF中MMP-9和TIMP-1的表达明显高于正常组(P<0.05和P<0.01);COPD组血清及BALF的MMP-9/TIMP-1低于正常组血清及BALF的MMP-9/TIMP-1,差异有统计学意义(P<0.05);COPD组和正常组BALF中MMP-9和TIMP-1水平高于血清中MMP-9和TIMP-1水平,但差异无统计学意义(P>0.05)。结论:MMP-9和TIMP-1平衡失调参与了COPD的发病,调节MMP-9和TIMP-1的平衡可能是治疗COPD的新方法。

Objective：To explore the expression of matrix metalloproteinase-9（MMP-9） and tissue inhibitor of metalloproteinase-1（TIMP-1） in airway of rat model of Chronic Obstructive Pulmonary Disease（COPD）.Methods：25 Wistar rats were randomly divided into COPD model group and normal control group.COPD rat model were established singly by passive smoking.Enzyme linked immunosorbent assay（ELISA）was used for determination of expression level of MMP-9 and TIMP-1 in the serum and bronchoalveolar lavage fluid（BALF） of two rat groups.Results：Expression level of MMP-9 and TIMP-1 in the serum and BALF of COPD group was significantly higher than that in the control group（P0.05andP0.01）;MMP-9/TIMP-1 in the serum and BALF of COPD group was lower compared to the normal control group,the difference was statistically significant（P0.05）;MMP-9 and TIMP-1 expression in the BALF was higher than that in the serum of both COPD group and the control group,but the difference was not statistically significant（P0.05）.Conclusions：An imbalance of MMP-9 and TIMP-1 regulation may be involved in the pathogenesis of COPD.Keeping the right balance between MMP-9 and TIMP-1 may be a new option for treatment of COPD.