加味生脉饮注射液对内毒素休克大鼠脑组织肿瘤坏死因子-α、白细胞介素-1α含量的影响

Impact of Supplemented Shenmaiyin Injection on Contents of TNF-α、IL-1α in Brain Tissue of Endotoxin Shock Rats

目的:以加味生脉饮注射液对内毒素休克大鼠脑组织TNF-α、IL-1α含量的影响为切入点,探讨补气活血解毒治法的作用机制。方法:96只SD大鼠随机分为假手术组(生理盐水20mL/kg)、模型组(脂多糖8mg/kg)、加味生脉饮组(脂多糖8mg/kg+加味生脉饮注射液10mL/kg)、地塞米松组(脂多糖8mg/kg+地塞米松5mg/kg)4组,每组再分为1h、2h、3h和6h4个观测时点。各动物手术分离颈总动脉并与生物机能系统连接,监测平均动脉压(MAP),测定脑组织TNF-α、IL-1α含量等指标。结果:模型组动物在注射脂多糖后MAP进行性下降,TNF-α、IL-1α含量增加(P<0.05),各治疗组上述病理改变明显改善。结论:生脉饮加味方可通过对休克时脑内过量炎性递质生成的抑制而起到抗休克、保护脑组织的作用,补气活血解毒治法是感染性休克有效的中医治法。

Objective：To investigate impact of Supplemented Shenmaiyin Injection on contents of TNF-α、IL-1α in brain tissue of endotoxin shock rats so as to discuss mechanism of the method of qi-invigorating,blood-promoting and detoxification.Methods：SD rats（n=96） were randomly divided into 4 groups,i.e. sham operated group （saline 20ml/kg）,model group（lipopolysaccharide LPS 8mg/kg）,Shenmaiyin group （LPS 8mg/kg＋Injection of reforming Shenmaiyin 10ml/kg）,and dexamethasone group （LPS 8mg/kg＋ dexamethasone 5mg/kg）,each group subdivided to be detected at 1h,2h,3h and 6h hour point.All subjects were operated to separate carotid artery and connected with biological functional systems to monitor mean arterial pressure（MAP） and determine TNF-α、IL-1α levels in brain tissues.Results：MAP of model group drawdown after injection of LPS,increase of the content of TNF-α、IL-1α in brain tissue were observed at the same time,above-mentioned pathological changes were improved obviously in each treatment groups. Conclusion：By way of inhibiting excess mediators of inflammation in brain tissue of endotoxin shock,Supplemented Shenmaiyin contribute to antishock and brain protection,method of qi-invigorating,blood-promoting and detoxification is effective infective shock.