脑得生丸中红花提取物纯化工艺研究

Study on Extraction and Purification of Safflower in NaoDeSheng Pills

目的:研究大孔吸附树脂分离纯化脑得生丸中红花的工艺,确定最佳工艺条件和参数。方法:红花水提取液浓缩后过大孔吸附树脂柱,以羟基红花黄色素A的含量为考察指标,对影响羟基红花黄色素A分离纯化的工艺参数进行考察。结果:HPD-100型树脂为红花最佳分离纯化树脂,其分离纯化红花的最佳工艺条件:吸附流速1mL·min-1,最大上样量为5倍树脂柱体积,洗脱剂为30%乙醇,洗脱流速为3mL·min-1等。结论:HPD-100型树脂在所确定的工艺条件下能很好地分离纯化羟基红花黄色素A,为纯化红花的最佳工艺。

Objective：To optimize the extraction and purification procedure of safflower in Naodesheng Pills by macroporous adsorptive resins. Methods：Safflower was extracted by water and depurated by macroporous adsorptive resins,the content of hydroxysafflor yellow A were used as indices of extraction and purification,inspect the factors that may affect the extraction rate of hydroxysafflor yellow A. Results： The optimal conditions of the purification of safflower were as follows：the water-extraction of safflower was depurated by HPD-100,the volume of sample was five times as much as macroporous adsorptive resins with adsorption rate of 1mL/min,30% alhocol was used as eluant and 3mL/min. Conclusion：The optimized extraction and purification procedure is the better one which can provide experimental basis for industrial production.