庆大霉素诱导急性肾损伤大鼠模型肾损伤分子-1的表达

Expression of Kidney Injury Molecule-1 in Rat Model of Acute Kidney Injury Induced by Gentamycin

目的探讨庆大霉素(Gentamycin,GM)诱导急性肾损伤大鼠模型肾损伤分子-1(Kidney injury molecule-1,KIM-1)的表达。方法建立GM诱导大鼠急性肾损伤模型;检测各组大鼠血、尿各项生化指标;取大鼠肾组织,进行病理组织学观察;ELISA法检测各组大鼠尿液中KIM-1的分泌;RT-PCR法检测各组大鼠肾组织KIM-1mRNA的表达;免疫组织化学SP法检测各组大鼠肾组织KIM-1、α-平滑肌肌动蛋白(α-SMA)、波形蛋白(Vimentin)的表达。结果与对照组比较,模型组大鼠血、尿各项生化指标及病理组织学观察均出现不同程度的病变;ELISA检测显示,大鼠尿液中KIM-1的含量显著增高(P<0.05);RT-PCR检测显示,肾组织KIM-1mRNA表达上调(P<0.05),且呈时间依赖性;免疫组化法检测显示,KIM-1表达量随肾损伤加重而显著升高,且呈时间依赖性。结论 KIM-1具有良好的敏感性和特异性,可作为GM所致肾小管损伤的早期诊断标志物。

Objective To investigate the expression of kidney injury molecule-1（KIM-1）in rat model of acute kidney injury induced by gentamycin（GM）.Methods Rat model of acute kidney injury induced by GM was established.Using those of normal rats as control,the biochemical indexes in blood and urine of model rats were determined,the kidney tissue was observed for histopathology,the secretion of KIM-1 in urine was determined by ELISA,the transcription of KIM-1 mRNA in kidney tissue by RTPCR,and the expressions of KIM-1,α-smooth muscle actin（SMA）and vimentin in kidney tissue by immunohistochemical assay with SP staining.Results Compared with those in control group,the biochemical indexes in blood and urine as well as histopathological observation of kidney tissues of model rats indicated pathological changes at various degrees;ELISA showed that the KIM-1 content in urine of model rats increased,and RT-PCR proved that the transcription level of KIM-1 mRNA in kidney tissue of model rats was upregulated（P 0.05）,both in time-dependent modes.Immunohistochemical assay showed significant time-dependent increase of expression level of KIM-1 with the increasing severity of kidney injury.Conclusion KIM-1 may be used as a sensitive and specific marker for early diagnosis of kidney injury induced by GM.