融合蛋白IFNα2a-α-MSH对小鼠黑色素瘤生长的抑制作用

Inhibitory Effect of Fusion Protein IFNα2a-α-MSH on Growth of Melanoma in Mice

目的观察融合蛋白IFNα2a-α-MSH对小鼠移植黑色素瘤生长的抑制作用。方法将小鼠黑色素瘤细胞株B16接种于C57BL/6小鼠背部皮下,待肿瘤直径长至4mm以上时,将小鼠分为4组,分别经肌肉注射生理盐水(阴性对照)、IFNα2a(9×106U/kg鼠重)、IFNα2a-α-MSH(9×106U/kg鼠重)及腹腔注射环磷酰胺(20mg/kg鼠重,阳性对照),前3组1次/d,阳性对照组隔日1次,共11d。隔日测量肿瘤大小;末次给药后24h,处死小鼠,称瘤重,并计算肿瘤抑制率。结果 IFNα2a-α-MSH可明显抑制小鼠黑色素瘤的生长,且其抑瘤作用明显强于IFNα2a。结论 IFNα2a-α-MSH对小鼠黑色素瘤生长有较强的抑制作用,为IFNα2a-α-MSH临床治疗黑色素瘤提供了实验依据。

Objective To observe the inhibitory effect of fusion protein IFNα2a-α-MSH（α-melanocyte-stimulating hormone） on the growth of transplanted melanoma in mice.Methods Melanoma cell strain B16 was inoculated s.c.to C57BL /6 mice.After the diameters of tumors were more than 4 mm,the mice were divided into four groups.The mice in group 1~3 were injected i.m.with physiological saline（negative control）,IFNα2a（9 × 106 U /kg body weight）and IFNα2a-α-MSH（9 × 106 U /kg body weight）respectively,once a day for 11 d,while those in group 4 were injected i.p.with cyclophosphamid（20 mg /kg body weight,positive control） every other day for 11 d.The sizes of tumors were measured every other day.The mice were killed 24 h after the last injection,and their tumors were weighed,based on which the inhibitory rate to growth of tumors was calculated.Results IFNα2a-α-MSH inhibited the growth of melanoma in mice significantly,and its inhibitory effect was significantly stronger than that of IFNα2a.Conclusion IFNα2a-α-MSH showed strong inhibitory effect on the growth of melanoma in mice,which provided an experimental basis for the clinical therapy of melanoma with IFNα2a-α-MSH.