大白菜遗传图谱的构建及与染色体关联分析

Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage

为构建大白菜遗传图谱,并与国际上公认的白菜参照图谱相关联。以大白菜晚抽薹DH系Y177-12和早抽薹DH系Y195-93为亲本建立的183个DH系为作图群体,利用SRAP、SSR、AFLP、STS、ESTP和CAPS等多种分子标记和形态标记构建大白菜遗传图谱,通过参照图上的锚定标记确定连锁群,并用最新的连锁群命名法进行命名。构建了包含10个连锁群、233个标记位点的分子遗传图谱,其中包括145个SRAP标记、62个SSR标记、12个AFLP标记、6个STS标记、4个ESTP标记、3个形态标记和1个CAPS标记。图谱总长度1 036 cM,标记间的平均图距为4.4cM。通过其中的38个SSR,4个ESTP和2个STS标记将各个连锁群与国际上认可A1-A10的参照连锁群相关联。该图谱的构建将为不同图谱的比较与整合及重要农艺性状的基因定位奠定基础。

The objective of the present study is to construct a genetic linkage map associated with internationally agreed reference linkage map in Chinese cabbage（Brassica rapa L.ssp.pekinensis）.183 DH lines derived from a cross of two DH lines,Y177-12 and Y195-93,were used for map construction.Six kinds of marker systems including SRAP,SSR,AFLP,STS,ESTP and CAPS loci were used for polymorphic marker screening.The assignment of linkage groups to their chromosomes was determined by using markers which have been mapped in the reference map.A genetic linkage map was constructed for Chinese cabbage,covering 1 036 cM,with an average mapping interval of 4.4 cM.233 loci including 145 SRAP,62 SSR,12 AFLP,6 STS,4 ESTP,1 CAPS and 3 morphological markers were inte-grated into 10 linkage groups using JoinMap 3.0.38 SSRs,4 ESTPs and 2 STSs provided anchors to previously pub-lished linkage map for B.rapa and B.napus and the consensus nomenclature of A1-A10 was adopted in this study.A genetic linkage map of Chinese cabbage associated with chromosomes was constructed,which enabled the alignments and integration between sets of maps and populations and would be useful in gene localization,comparative genomes research and QTL mapping of important agronomic traits for Chinese cabbage.