抗人GPC3单克隆抗体在肝脏肿瘤组织学检测中的应用

Application of anti-GPC3 monoclonal antibody in examination of hepatocellular tumors

目的应用抗人GPC3单克隆抗体检测肝脏良、恶性肿瘤组织中GPC3蛋白的表达。方法采用自制鼠抗人GPC3单克隆抗体及免疫组织化学技术检测19例正常肝组织、94例肝细胞癌(HCC)组织、20例胆管细胞癌组织、42例肝脏良性肿瘤组织中GPC3蛋白的表达,并分析GPC3蛋白表达水平与HCC患者临床病理参数的关系。结果 GPC3蛋白在肝细胞癌组织中阳性率达到79.8%,而血管瘤旁正常肝组织、胆管细胞癌组织和肝脏良性肿瘤组织中均不表达,差异具有统计学意义(P<0.001)。HCC组织中GPC3蛋白的表达与血清AFP水平(P=0.020)、是否伴有门静脉癌栓(P=0.050)、有无包膜等因素相关(P=0.043)。结论抗人GPC3单克隆抗体可特异性检出肝细胞癌组织GPC3蛋白的表达,值得进一步研究以应用于临床诊治。

Objective To evaluate the feasibility of using anti-GPC3 monoclonal antibody in discriminating benign and malignant hepatocellular lesions.Methods The expression of GPC3 was examined by semiquantitative immunohistochemistry assessment in 19 normal control samples,94 hepatocellular carcinoma samples,20 intrahepatic cholangiocarcinoma samples and 42 benign hepatocellular samples.The relation of GPC3 expression profile with the clinicopathological factors of HCC was analyzed.Results GPC3 staining was positive in 75（79.8%）of the 94 HCC samples.And GPC3 expression was not detected in normal liver tissue adjacent to hepatic hemangioma,intrahepatic cholangiocarcinoma,or benign hepatic lesions.GPC3 protein expression in HCCs was correlated with serum alpha-fetoprotein level（P=0.020）,tumor encapsulation（P=0.043）,and portal vein invasion（P=0.050）.Conclusion Anti-human GPC3 mouse monoclonal antibody can specifically detect GPC3 protein expression in hepatocellular carcinomas tissues,which warrants further examination for future clinical application.