紫荆花黄酮类化合物体外抗氧化活性研究

Study on Antioxidant Activities of Total Flavonoids from Bauhinia Blakeana in Vitro

采用Fenton反应体系产生羟自由基和邻苯三酚自氧化产生超氧阴离子自由基,研究了紫荆花黄酮类化合物对两者的清除作用。并以芦丁为对照品,测定了其总黄酮含量。结果表明:紫荆花中总黄酮含量为9.094 mg/g。紫荆花黄酮类化合物对羟自由基和超氧阴离子自由基均有清除作用,其浓度的添加量在试验范围内与其抗氧化活性呈正相关。紫荆花黄酮类化合物对羟自由基半数清除量(IC50)为127.822μg/mL。

The scavenging effects of total flavonoids from bauhinia blakeana dunn on hydroxyl free radical （·OH） produced by Fenton reaction and superoxide anion free radical （O2^-·） produced by oxidation of pyrogallol were studied.The results showed that the content of total flavonoids in bauhinia blakeana dunn determined by spectrophotometry is 9.094 mg/g,and the flavonoids can both scavenge hydroxyl free radical （·OH） and superoxide anion free radical （O2^-·）.The dosage of the extracts is in direct proportion to the antioxidation activity,and their inhibitory concentration of 50%（IC50） is 127.822 μg/mL.