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低温诱导甜菜(Beta vulgaris L.)抽薹相关基因的RACE分析 被引量：4

RACE analysis of bolting related genes in cold induced sugar beet(Beta vulgaris L.)
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摘要 采用cDNA的末端快速扩增(Rapid amplificationn of cDNA ends)技术,以代表性差异分析cDNA-RDA(Representational difference analysis of cDNA)技术获得的低温诱导甜菜茎尖中差异表达的基因片段为模板,进行巢式PCR扩增,然后根据RACE拼接结果合成引物,分别以低温诱导的cDNA和DNA为模板进行PCR扩增,克隆测序后获得1个全长609 bp的cDNA和855 bp的DNA,分别命名为Ty7Br600和Ty7Br900,在GenBank注册,登录号分别为AY324115和AY324114。在GenBank库中的核苷酸序列比较未发现它们的同源序列,因此认为这个cDNA序列可能是甜菜的一个新基因。 cDNA-RDA was applied to clone genes specifically expressed in cold induced sugar beet. One cDNA fragment was obtained after three rounds of subtractive hybridization and amplified by 3＇ RACE and 5＇ RACE. A sequence of 609 bp cDNA named Ty7Br600 and 855 bp DNA named Ty7Br900 were obtained by RT-PCR and PCR, respectively after cloning and sequencing. The accession numbers were AY324115 and AY324114 in GenBank database, respectively. Blasting in GeneBank showed no homology with them, suggesting that it might be a novel gene of sugar beet.
作者 戴建军 常缨 李彩凤 马凤鸣
机构地区 东北农业大学资源与环境学院 东北农业大学生命科学学院 东北农业大学农学院
出处 《东北农业大学学报》 CAS CSCD 北大核心 2010年第7期10-15,共6页 Journal of Northeast Agricultural University
基金 黑龙江省教委研究课题(10531025)
关键词 甜菜 低温诱导 cDNA—RDA 基因克隆 序列分析 sugar beet cold induction cDNA-RDA gene cloning sequence analysis
分类号 S566.3 [农业科学—作物学] Q785 [生物学—分子生物学]
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参考文献16

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二级参考文献12

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二级引证文献7

东北农业大学学报
2010年 第7期

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