黑龙江省大蒜病毒病原鉴定及病毒病发生情况调查

Study on pathogen identification and virus disease investigation of garlic virus in Heilongjiang Province

黑龙江省大蒜病毒病的田间症状复杂,研究通过生物学技术、电子显微镜技术和血清学方法相结合对大蒜病毒病原进行鉴定,并对黑龙江省大蒜病毒病的发生情况进行了调查。结果表明,表现花叶症状的大蒜样品中含有韭葱黄条病毒(Leek yellow strape virus,LYSV)和大蒜潜隐病毒(Garlic latent virus,GarLV);表现黄化症状的大蒜样品中含有洋葱黄矮病毒(Onion yellow dwarf virus,OYDV)和大蒜普通潜隐病毒(Garlic common latentvirus,GCLV);表现卷叶症状的大蒜样品中含有GCLV、LYSV和OYDV。黑龙江省大蒜病毒病的发生相当严重,且多数为复合侵染,其中LYSV、GCLV和GarLV的检出率较高。

The field symptom of garlic virus is complex in Heilongjiang Province, by using biological method, electron microscope and serology method to identify pathogen of garlic virus, and at the same time, investigate the garlic viruses in Heilongjiang Province. The results showed that the garlic sample of mosaic symptoms contained LYSV and GarLV; the garlic sample of etiolation symptoms contained OYDV and GCLV; the garlic sample of leaf roll contained GCLV, LYSV and OYDV. It was very severe garlic virus in Heilongjiang, and moreover complex infection. Among of them, LYSV, GCLV and GarLV were the main viruses.