黑曲霉LZ1降解咪唑乙烟酸的特性

Degradation of imazethapyr by Aspergillus sp. LZ1

黑曲霉LZ1是从长期施用咪唑乙烟酸的大豆田土壤中分离得到的1株降解真菌,它能够以咪唑乙烟酸为唯一碳源进行生长,在含200 mg.L-1咪唑乙烟酸的基础盐培养液中,8 d降解率可达72.5%。以黑曲霉LZ1的菌体生长量和咪唑乙烟酸降解率为指标,研究了影响生长和降解的主要因素。结果表明,黑曲霉LZ1生长和降解咪唑乙烟酸的适宜条件是:pH 5～7,温度25～35℃,咪唑乙烟酸浓度50～300 mg.L-1,接种量(V/V)≥2%。

Aspergillus sp. LZ1, a strain of degradable fungus isolated from soybean field contaminated with imazethapyr for a long time, could grow utilizing imazethapyr as the sole carbon source. When the strain spore was inoculated into imazethapyr-inorgan salt medium at 200 mg .L1, the degra- dation rate reached 72.5% in 8 d. In the experiment, the influencing factors was studied using fungus growth mass and degradation rate as indexes and the results showed that the suitable conditions for the fungus growth and imazethapyr degradation were as follows： pH 5-7, temperature 25-35 ℃, imazethapyr concentration 50-300 mg .L-1, and inoculation rate should be no less than 2%（VN）.