CK7和CK20在肝内胆管癌及肝内胆管结石旁胆管组织中的表达

Expression of CK7/CK20 in intrahepatic cholagiocarcinoma and hepatolithiasis

目的:探讨细胞角蛋白7(CK7)、细胞角蛋白20(CK20)在肝内胆管癌及肝内胆管结石旁胆管组织中的表达及临床意义。方法:免疫组织化学方法检测30例肝内胆管癌、例肝内胆管结石旁胆管组30织及15例正常肝内胆管组织中的CK7、CK20表达。结果:CK7在肝内胆管癌、正常肝内胆管组织中的阳性表达率分别为96.7%(29/30)、13.3%(2/15),差异有统计学意义(P<0.01);CK20在肝内胆管癌组织、正常肝内胆管组织中阳性表达率分别为43.3%(13/30)、(1/15),差异有统计学意义(P<0.01)。CK76.7%在肝内胆管结石旁胆管组织阳性表达率为76.6%(23/30),与正常肝内胆管组织阳性表达率13.3%(2/15)比较,差异有统计学意义(P<0.05)。CK20在肝内胆管结石旁胆管组织阳性表达率为6.7%(2/30),与正常肝内胆管组织阳性表达率6.7%(1/15)比较,差异无统计学意义(P>0.05)。结论:CK7和CK20可能成为肝内胆管癌特异性肿瘤标志物。

Objective：To study the expression of CK7/CK20 in intrahepatic cholangiocarcinoma and hepatolithiasis.Methods：CK7/CK20 were analysed in 30 cases of intrahepatic changiocarcinoma and 30 cases of hepatolithiasis by immunohistochemical method.Results：The positive rates of CK7 expression were 96.7%（29/30）in intrahepatic cholangiocarcinoma and 13.3%（2/15）in normal intrahepatic bile duct respectively.Their difference was significant（P0.01）.The positive rates of CK20 expression were 43.3%（13/30）in intrahepatic cholangiocarcinoma,and 6.7%（1/15）in normal intrahepatic bile duct respectively.Their difference was significant（P0.01）.The positive rates of CK7 expression were 76.6%（23/30）in hepatolithiasis and 13.3%（2/15）normal intrahepatic bile ductrespectively.Their difference was significant（P0.05）.The positive rates of CK20 expression were 6.7%（2/30）in hepatolithiasis and 6.7%（1/15）in normal intrahepatic bile ductrespectively.Their difference was no significant（P0.05）.The expression of CK7/CK20 does not correlate with the sex and age of patients in intrahepatic changiocarcinoma and tumor＇s histological grading（P0.05）.Conclusion：CK7/CK20 may be tumor makers of intrahepatic cholangiocarcinoma.