脂质体包裹鼠疫菌VW_a^-EV_(76)paris株家兔免疫血清的体外杀菌作用研究

Bactericidal activity in vitro of rabbit immune serum against VW aEV 76 paris coated liposome

目的观察清除了４５ＭＤ质粒的巴黎ＥＶ７６菌株的家兔免疫血清的体外杀菌作用。方法家兔行背部皮下注射免疫；Ｆ１抗体滴度用间接血凝试验标定；体外杀菌试验采用平板活菌计数。结果用脂质体包裹的ＶＷａＥＶ７６ｐａｒｉｓ株免疫家兔，家兔体内可产生杀菌性抗体，该抗体的杀菌效果明显优于单独以ＥＶ兰活菌苗的血清抗体，但在接种部位易形成肉芽肿病灶。结论脂质体包裹的ＶＷａＥＶ７６ｐａｒｉｓ家兔免疫血清具有较强的杀菌作用。

Objectives To observe the bactericidal activity in vitro of rabbit immune serum against VW aEV 76 paris coated liposome.Methods The titer of antibody F 1 was tested in indirect hemagglutination.And the viable bacteria counts in medium were caculated as the index of serum bactericidal activity in vitro.Results The rabbits injected VW aEV 76 paris was able to produce antibody against Y.pestis. The antibody had stronger bactericidal activity than that of the ordinary alive EV vaccine.Conclusion The rabbit immune serum against VW aEV 76 paris coated liposome has stronger bactericidal activity