摘要
应用ＰＣＲ技术和小型聚丙烯酰胺凝胶电泳银染色检测法，对云南白族人群和云南汉族人群Ｄ１７Ｓ３０，Ｄ１Ｓ８０和ＡｐｏＢ基因座遗传多态性进行了调查．发现白族人群Ｄ１７Ｓ３０基因座有１１个等位基因，其频率范围为０００２７～０１８１８；Ｄ１Ｓ８０基因座有１９个等位基因，频率范围在０００４３～０２４１４．汉族人群Ｄ１７Ｓ３０基因座有１２个等位基因，其频率范围为０００４８～０１９２０；Ｄ１Ｓ８０基因座有１８个等位基因，频率范围为０００３０～０２６３３；ＡｐｏＢ基因座有１２个等位基因，频率范围为０００３９～０４６４８．
The polymorphism distribution of loci D 17 S 30 , D 1S 80 in the Bai Ethnic group and loci D 17 S 30 , D 1S 80 and ApoB in Han people in Yunnan was studied in PCR, PAGE and silver staining. The results showed that the alleles (range of genes frequencies) of locus D 17 S 30 and locus D 1S 80 were 11 (0.002 7～0.181 8) and 19 (0 004 3～0 241 4) in the Bai Ethnic group. The genes (and genes frequencies) of loci D 17 S 30 , D 1S 80 and ApoB were 12 (0 004 8～0 219 0), 18 (0 003 0～0 263 3) and 12 (0 003 9～0 464 8) in Han people. Also, the data of the discrimination power (DP) and the heterozygosity of the reciprocal locus on two people were obtained in this study.
出处
《昆明医学院学报》
1999年第1期4-9,共6页
Journal of Kunming Medical College
基金
云南省应用基础研究基金
关键词
D17S30基因
D1S80基因
APOB基因
遗传多态性
D 17 S 30 Locus, D 1S 80 locus, ApoB locus, Genetic polymorphism, Allele frequency, Amplified fragment length polymorphism , Discrimination power
