LBP_2对大鼠免疫功能影响的体外实验研究

Effect of LBP 2 on Functions of Rats Peritoneal Macrophages in Vitro

研究了ＬＢＰ２（ｌｙｃｉｕｍｂａｒｂａｒｕｍｐｏｌｙｓａｃｃｈａｒｉｄｅ－２）对ＳＤ大鼠腹腔巨噬细胞（ＭΦ）功能的影响，以探讨ＬＢＰ２的免疫调节机制．ＭΦ吞噬中性红试验研究表明，ＬＢＰ２在体外具有浓度（０３２～２０ｍｇ／Ｌ）依赖性增强大鼠腹腔ＭΦ吞噬中性红的能力．另外，采用生物活性检测法测定了细胞因子的水平，将大鼠ＭΦ用ＬＰＳ（１０ｍｇ／Ｌ）和ＬＢＰ２共同孵育１８～２４ｈ后，培养上清及细胞内ＩＬ－１和ＴＮＦ水平均随ＬＢＰ２浓度的增加而增加，表明ＬＢＰ２对ＭΦ合成和释放ＩＬ－１，ＴＮＦ具有明显促进作用．ＬＢＰ２对ＭΦ功能的影响可能是其免疫调节作用的机制之一．

This research explored the effect of LBP 2 (Lycium Barbarum Polysaccharide 2) on functions of SD rats peritoneal macrophages (MΦ) and the possible immunomodulatory mechanism of LBP 2. Neutral red phagocytized by MΦ represented phagocytic activity. LBP 2 enhanced the activity of rats peritoneal macrophages to phagocytize neutral red in vitro with dose increased (0.32～20 mg/L). Moreover, the standard of cytokines was examined by the methods of biological activity. LBP 2 was shown to significantly induced intracellular and extracellular production of interleukin-1 (IL-1) and tumornecrosis factor (TNF) with LPS (10 mg/L) in 18～24 hours. LBP 2 increased the synthesis and release of IL-1, TNF from rats peritoneal MΦ in vitro. Therefore, the effect of LBP 2 on the production of IL-1, TNF from MΦ might be the main immunomodulatory mechanism of LBP 2 .