幽门螺杆菌空泡毒作用及空泡毒中和试验

Vacuolationg inoculated by helicobacter pylori and neutralization assay

目的探讨幽门螺杆菌（ｈｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉ，Ｈｐ）的空泡毒作用及其与疾病的关系。方法用细胞培养观察Ｈｐ的空泡毒作用。结果６９．３５％（４３／６２株）的Ｈｐ空泡毒作用阳性，其空泡毒作用能被相应患者血清中和。７８．２６％（３６／４６例）的消化性溃疡患者，感染的是空泡毒作用阳性的Ｈｐ＋（Ｔｏｘｉｎ＋），而只有４２．８６％（６／１４例）胃炎患者感染的是Ｈｐ＋，两者Ｈｐ＋感染率差异有显著性（χ２＝４．８３，Ｐ＜０．０５）。结论Ｈｐ＋感染与消化性溃疡密切相关，其在体内也有空泡毒作用并产生相应抗体。

Objective To study the vacuolation of Helicobacter pylori (Hp) and its relation with disease. Methods Cytoculture was used to study the vacuolation induced by Hp. Results 69.35% (43/62 strains) of Hp was vacuolated and could be neutralized by serum from related patients. 78.26% (36/46 cases) of patients with peptic ulcer were infected with Hp + (Toxin+) strains, and only 42.86% (6/14 cases) of patients with gastritis were infected with Hp + strains. There (χ 2=4.83, P <0.05). Conclusions Infection with Hp + (Toxin+) was more often associated with peptic ulcer disease. Hp + was vacuolated and produced antibodies in vivo.