肺癌膜蛋白识别分子与抗肿瘤反应的初步研究

Preliminary study on immunomolecules against tumor cell membrane proteins in patient with lung cancer: Association with antitumor response

目的探讨肺癌血清中肿瘤自身识别和抗肿瘤反应物质。方法取肺鳞癌、腺癌、大细胞癌、小细胞未分化癌术后新鲜组织匀浆，离心，超声粉碎提取胰蛋白，与溴化氰活化的Ｓｅｐｇｈａｒｏｓｅ４Ｂ偶联，通过免疫亲合层析分离血清中肺癌胰蛋白识别分子，并对其理化特性和免疫学特性进行了初步研究。结果肺癌血清中存在识别肺癌胰蛋白的活性分子，其相对分子质量约为７００００、６６０００、１５２００；经特异蛋白分析仪分析，提示为一类与抗体无关的免疫识别分子；细胞抑制试验证实，在１０～４０１ｍｇ／Ｌ（终浓度）时，能够抑制ＧＬＣ－８２细胞生长，抑制率为２４．４％～３７．２％；细胞毒试验证实，在５～２０ｍｇ／Ｌ（终浓度）时能够提高ＬＡＫ细胞对ＧＬＣ－８２细胞杀伤活性，杀伤率为２８．３１％～２８．８８％，对照组为１５．６３％（Ｐ＜０．０５）。结论提示肺癌病人血清中存在肿瘤免疫识别物质和抗肿瘤免疫反应，并且具有双向调控肿瘤细胞生长的作用。

Objective In order to evaluate the significance of immunomolecules against lung cancer associated with immunorecognition in diagnosis and therapy of neoplasms. Methods The membrane proteins of lung cancer tissues were extracted andcoupled with CNBr-activated Sepharose 4B according to the routine method. By immune affinity chromatography. a kind ofmolecules was puritied and analyzed by SDS-PAGE, and cell inhibition and cytotoxicity tests in vitro. Results A kind of molecules0ecognizing membrane proteins of lung cancer, not globulins, was purified with the molecular weight of 70 000,66 000,15 200.which can inhibit the growth of GLC-82 cells and enhance the cytotoxicity of LAK cells for target cells (P <0.05). Conclusion Thereare mediator of immunorecognition and antitumor response in the serum of lung cancer patients, which could bidirectionally controlgrowth of tumor cell.