胆囊结石患者胆汁需氧菌和厌氧菌DNA检测及意义

Measurement and its significance of DNA of aerobic and anaerobic bacteria in bile in gallstone patients

目的从分子生物学水平研究胆囊结石患者胆汁细菌脱氧核糖核酸（ＤＮＡ），探讨细菌在胆结石发病机理中的作用。方法采用聚合酶链式反应（ＰＣＲ）对胆汁中５种需氧菌和４种厌氧菌ＤＮＡ进行检测。结果检测７０例，存在需氧菌５６例（８０％）和厌氧菌ＤＮＡ２３例（３２．８６％）。两类细菌ＤＮＡ同时存在者为２０例（２８．５７％）。首次从２１例胆汁中检测出幽门螺杆菌ＤＮＡ。结论胆结石胆汁中存在多种细菌ＤＮＡ，胆结石核心可能与细菌感染有关。

Objective To study DNA of bacteria in bile at molecular biology level and explore the role of bacteria in gallstonepathogenesis. Methods DNA of 5 kinds of aerobic and 4 kinds ofanaerobic bacteria in bile was tested by polymerase chain reaction(PCR). Results DNA of the aerobic and anaerobic bacteria was detected in 56 (80%) and 23 patients (32.86%) in 70 patients testedrespectively DNA of the both kinds of the bacterium was detected in 20 patients (28.5o/o). DNA of Helicobacter pylori was detectedin 21 patients at first. Conclusion There are multiple kinds ofbacterial DNA in the bile and gallstone nuclear detected may be relatedto bacterial infection.