高效液相色谱法同时测定化妆品中尼泊金酯类防腐剂和特丁基对苯二酚

Determination of TBHQ and Nipagin Esters in Cosmetics Using High Performance Liquid Chromatography

为建立化妆品中防腐剂和抗氧化剂的分析方法,采用反相高效液相色谱法同时测定化妆品中4种尼泊金酯类防腐剂和特丁基对苯二酚（TBHQ）.Diamonsil C18（5μm,250 mm×4.6 mmi.d.）分离柱,甲醇-水（体积比72∶28）流动相,流速1.0 mL/min,室温,检测波长254 nm.4种尼泊金酯类防腐剂在1~100 ng/L内均有良好线性,检出限为0.05~0.1 ng/L,回收率为82.9%~96.3%,相对标准偏差（RSD）为1.17%~2.64%.

A method has been established for the analysis of presevatives and antioxidants in cosmetics.Reversed phase high performance liquid chromatography（RP-HPLC） was used for separation and determination of nipagin esters and TBHQ in cosmetics.The components were analyzed by Diamonsil C18（5 μm,250 mm×4.6 mm i.d.）using a UV detector at 254 nm.The mobile phase was methanol water（V∶V=72∶28） at a flow rate of 1.0 mL/min.The methodology exhibited good linearity between 1—100 ng/L.The detection limits range were 0.05—0.1 ng/L.The average recovery ranged from 82.9%—96.3% with RSD ranged from1.17%—2.64 %.