急性肾缺血再灌注大鼠肾细胞游离钙水平与细胞凋亡的研究

Studies of both intracellular ionized calcium level and apoptosis during acute renal ischemic reperfusion injury

目的观察缺血再灌注损伤对肾细胞游离钙([Ca2+]i)水平和细胞凋亡的影响。方法采用摘除左肾,钳夹大鼠右侧肾蒂,建立急性缺血再灌注肾损伤模型,应用Fura-2/AM荧光指示剂测定缺血再灌注大鼠肾细胞[Ca2+]i水平,流式细胞术检测肾细胞凋亡率。结果缺血再灌注不同时期肾细胞呈现不同程度Ca2+超载,与对照组相比差异显著(P<0.01);肾细胞凋亡率增高,与对照组相比差异显著(P<0.05、P<0.01),有正相关趋势。结论缺血再灌注不同时期肾细胞游离钙([Ca2+]i)水平增高,并与肾细胞凋亡率有正相关趋势,再灌注时间与肾细胞Ca2+超载呈正相关,提示再灌注损伤在加重细胞钙超载同时增加了肾组织细胞凋亡,可能是再灌注损伤肾功能障碍的重要原因。

Objective Effects of renal ischemic reperfusion injury on intracellular inonized calcium level and apoptosis are observerd.Methods The model of rat＇s acute renal ischemic reperfusion injury was established by both cutting the left kidney and occlusing right kidney pedicle.The renal intracellular i level and the renal cell apoptosis were measured by using Fura-2/AM fluorescence assay and flow cytometry respectivly.Results Compared with the control group,renal intracellular [Ca^2＋]i level increased significantely（P〈0.01） and apoptosis rate increased significantely（P〈0.05,P〈0.01） at different periods of ischemic reperfusion.Conclusion At different periods of rat＇s acute renal ischemic reperfusion injury,renal intracellular I rises and becomes postive relationship with the apoptosis rate.There is positive relevent between the reperfusion time and renal intracellular i overloading.This may be important reason of renal disfunction due to reperfusion injury.