异丙酚预处理对大鼠肝缺血再灌注细胞因子的影响及肺损伤的保护作用

Effects of propofol pretreatment on cytokines and protection on hepatic ischemia-reperfusion-induced lung injury in rats

目的探讨异丙酚对肝缺血再灌注时TNF-α、IL-1含量的影响及其对肺损伤的保护作用。方法 24只SD大鼠随机分为假手术组(s组,n=8)、缺血再灌注组(I/R组)和异丙酚处理组(P组)。阻断肝门30 min后开放血流,建立大鼠全肝缺血再灌注模型。I/R组与p组分别于肝门阻断前10 min腹腔注射异丙酚50 mg/kg或相应剂量生理盐水,于再灌注1 h取血、处死动物。假手术组不阻断肝门,于上述相应时间点注射生理盐水与取血、处死动物。放免法测定血清TNF-α、IL-1的变化,光镜下观察肺组织形态学改变,同时测定肺组织W/D比值。结果 (1)与s组相比,I/R组血清TNF-αI、L-1含量与肺组织W/D比值均显著增加(P均<0.01)。病理学方面,I/R组肺泡腔完整性破坏,间隔增厚,并可见中性粒细胞浸润。(2)与I/R组相比,P组病理学改变减轻;W/D比值、血清TNF-αI、L-1含量均显著降低(P均<0.05)。结论 TNF-aI、L-1β等细胞因子参与了肝缺血再灌注后肺损伤的发生发展过程,丙泊酚可能通过抑制炎性细胞因子的表达,对大鼠肝I/R后肺损伤发挥有保护作用。

Objective To explore the effects of propofol on tumor necrosis factor-alpha（TNF-α）,interleukin-1（IL-1） in rats following hepatic ischemia/reperfusion and the protection of propofol on hepatic ischemia–reperfusion-induced lung injury.Methods 24 rats were randomly divided into 3 groups（n=8 in each group）：propofol（P） group,ischemia-reperfusion（I/R） group and sham-operation（S） group.Total hepatic I/R was produced by occlusion of hepatic helium for 30 minutes,and the occlusion was then released for reperfusion.In P and I/R group,propofol（50 mg/kg）or normal saline of the same volume was administered intraperitoneally 10 min before ischemia,the animals were killed at 1h of reperfusion.In S group,the hepatic helium wasn＇t occluded,normal saline was injected and the animals were killed at corresponding time.The concentration of IL-1,TNF-αin serum was determined,and the lung tissue was taken for determination of W/D ratio and histological examination.Results（1）Compared with that in sham-operation group,the concentration of IL-1,TNF-α,W/D ratio were all significantly increased（P〈0.01）.Histological examination revealed that the alveolar architecture was destroyed with interstitial thickening and neutrophil infiltration in total hepatic I/R group.（2）Compared with that in I/R group,the concentration of IL-1,TNF-α,W/D ratio were all significantly decreased（P〈0.05=,and the histological changes was less severe in propofol group.Conclusion Propofol can effectively suppress the production and release of inflammatory cytokines and its protective effect might be mediated by anti-inflammation.